semiconductor-based influenza a research microarray  (CombiMatrix)

Journal: BMC Biotechnology

Article Title: Multiplexed, rapid detection of H5N1 using a PCR-free nanoparticle-based genomic microarray assay

doi: 10.1186/1472-6750-10-74

Figure Lengend Snippet: Simplified schematic overview of the gold nanoparticle (NP)-based microarray assay . The target is whole viral genome RNA (blue line), end modified capture oligos are 40 bp in length (black line) printed on the amino-modified glass slide and intermediate oligos are 65 bp in length (purple line) including 25-mer poly (A) tail (red line). The probe is 15 nm gold nanoparticles (NP) functionalized with poly dT tail (green line). The specific hybridizations between the capture oligo, RNA (or DNA) and intermediate oligo take place to form sandwich complexes in Step 1, then the gold NP probes bind to the complexes in Step 2, and silver staining is in Step 3.

Article Snippet: CombiMatrix Corporation has also developed a semiconductor-based Influenza A Research Microarray that can detect all known subtypes of influenza A viruses within 5 hours [ ].

Techniques: Microarray, Modification, Silver Staining

Journal: BMC Biotechnology

Article Title: Multiplexed, rapid detection of H5N1 using a PCR-free nanoparticle-based genomic microarray assay

doi: 10.1186/1472-6750-10-74

Figure Lengend Snippet: Image from a Verigene ID™ detection system . (A). Microarray layout with positive control capture (closed red circles on the top), negative control which uses printing buffer as capture (closed yellow circles), M, H5, and N1 gene captures (filled as variable color of closed circles, spotted in triplicate) are indicated. (B). The light shades represent strong silver staining signals. A portion of the microarray images for DNA oligonucleotide following hybridization with PCR fragment are shown and light shades represent greater silver intensities for the M and H5 genes. The spots at the top of each array are positive controls. (C). Silver staining image for individual or multiple PCR products are shown and labeled. (D). Typical genomic microarray silver staining image from 0.2 μg of H1N1, H3N2 or H5N1 viral RNA are shown. The boxed areas highlight hits for specific subtypes.

Article Snippet: CombiMatrix Corporation has also developed a semiconductor-based Influenza A Research Microarray that can detect all known subtypes of influenza A viruses within 5 hours [ ].

Techniques: Microarray, Positive Control, Negative Control, Silver Staining, Hybridization, Labeling

Journal: BMC Biotechnology

Article Title: Multiplexed, rapid detection of H5N1 using a PCR-free nanoparticle-based genomic microarray assay

doi: 10.1186/1472-6750-10-74

Figure Lengend Snippet: Results of genomic array detection of influenza A viruses with specific captures

Article Snippet: CombiMatrix Corporation has also developed a semiconductor-based Influenza A Research Microarray that can detect all known subtypes of influenza A viruses within 5 hours [ ].

Techniques:

Journal: BMC Genomics

Article Title: Use of consensus sequences for the design of high density resequencing microarrays: the influenza virus paradigm

doi: 10.1186/1471-2164-11-586

Figure Lengend Snippet: Resequencing microarray output for identification and characterisation of the 12 influenza A viral RNAs

Article Snippet: Recently, Combimatrix Corporation (Mukilteo, WA) developed a semiconductor-based microarray and an integrated microfluidic array enabling the detection of 15 HA and 9 NA [ , ].

Techniques: Microarray

Journal: BMC Genomics

Article Title: Use of consensus sequences for the design of high density resequencing microarrays: the influenza virus paradigm

doi: 10.1186/1471-2164-11-586

Figure Lengend Snippet: Avian sequences reconstructed by the PathogenID v2.0 and BLASTN alignment

Article Snippet: Recently, Combimatrix Corporation (Mukilteo, WA) developed a semiconductor-based microarray and an integrated microfluidic array enabling the detection of 15 HA and 9 NA [ , ].

Techniques: Sequencing, Microarray